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Effect of CD44 and CD51 expression levels by BFP-4 treatment in BMSCs. BMSCs were treated with 0.1 μg/ml BFP-4. After 24 h, CD44 and CD51 expression were measured by flow cytometry (green arrow, ODM only; blue arrow, ODM plus BMP-7; pink arrow, ODM plus BFP4) (A) . Immunofluorescence analysis was conducted using anti-CD44 and anti-CD51 antibodies (B) . Data represent four independent experiments. Magnification, 400×.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Bone-Forming Peptide-4 Induces Osteogenic Differentiation and VEGF Expression on Multipotent Bone Marrow Stromal Cells

doi: 10.3389/fbioe.2021.734483

Figure Lengend Snippet: Effect of CD44 and CD51 expression levels by BFP-4 treatment in BMSCs. BMSCs were treated with 0.1 μg/ml BFP-4. After 24 h, CD44 and CD51 expression were measured by flow cytometry (green arrow, ODM only; blue arrow, ODM plus BMP-7; pink arrow, ODM plus BFP4) (A) . Immunofluorescence analysis was conducted using anti-CD44 and anti-CD51 antibodies (B) . Data represent four independent experiments. Magnification, 400×.

Article Snippet: At the end of 3 days incubation, the cells were treated by BFP-4 for 24 h. After 24 h incubation, cells were fixed with 4% paraformaldehyde prepared in PBS for 15 min, permeabilized with 0.1% Triton X-100 for 15 min, and blocked with 5% bovine serum albumin in PBS for 30 min. Coverslips were then incubated with a primary antibody against mouse CD44 and CD51 (eBioscience, San Diego, CA, United States) at a dilution of 1:200 followed by incubation with a secondary antibody at a dilution of 1:400, both at room temperature for 1 h. Cells were washed with PBS, and nuclei were counterstained with 4,6-diamidino-2-phenylindole.

Techniques: Expressing, Flow Cytometry, Immunofluorescence